The incident happened in Mdantsane, Eastern Cape, as Zolani Tete arrived at his residence and waited for the gate to open.
According to the police, two armed men wearing balaclavas stepped out of a vehicle and opened fire on the boxer.
A 27-year-old woman who was travelling with Tete was also shot several times and taken to hospital for treatment.
South Africa’s Minister of Sports, Arts and Culture, Gayton McKenzie, confirmed Tete’s death on Friday.
McKenzie expressed caution over speculation about the motive behind the attack, saying “I will not speculate about who did this or why.”
Police have launched an investigation, but no motive has been established and no details about possible suspects have been released.
Zolani Tete , who was nicknamed “Last Born”, became widely known in boxing for his remarkable 11-second knockout of fellow South African Siboniso Gonya in Belfast in November 2017.
The knockout remains one of the fastest stoppages in a world title fight.
Tete achieved it while defending his WBO bantamweight title, which he had won earlier that year and held until 2019.
The southpaw boxer had previously captured the IBF junior-bantamweight title in 2014 after defeating Japan’s Teiru Kinoshita in Kobe.
Earlier in his career, Tete had also held the minor WBF flyweight title.
Zolani Tete’s death came only weeks after he completed a four-year ban from boxing following a positive test for the banned anabolic steroid stanozolol after his 2022 bout against Jason Cunningham.
His suspension ended on July 29, and he had been preparing to make a return to professional boxing before his death.
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